KMID : 1007520120210030845
|
|
Food Science and Biotechnology 2012 Volume.21 No. 3 p.845 ~ p.852
|
|
Inhibition of ¥â-Amyloid Peptide-induced Neurotoxicity by Kaempferol 3-O-(6''-Acetyl)-¥â-glucopyranoside from Butterbur (Petasites japonicus) Leaves in B103 Cells
|
|
Song Kyung-Sik
Jeong Woo-Sik Jun Mi-Ra
|
|
Abstract
|
|
|
One of predominant hallmarks in Alzheimer¡¯s disease (AD) is extracellular senile plaques containing ¥â- amyloid peptide (A¥â). A¥â is known to be directly responsible for the free radical production and lipid peroxidation, leading to apoptosis and cellular death. In this study, we investigated the possible protective effect of kaempferol 3-O-(6''-acetyl)-¥â-glucopyranoside (KAG) isolated from butterbur (Petasites japonicus) leaves against A¥â- induced neurotoxicity. Exposure of mouse neuroblastoma B103 cells to A¥â(25-35) at the concentration of 50 ¥ìM significantly reduced cellular viability and increased both oxidative stress and apoptotic rate. However, pretreatment of B103 cell with isolated KAG at 10 ¥ìM significantly inhibited A¥â-induced apoptotic cellular damage and reactive oxygen species (ROS) generation. Pretreatment of KAG also completely inhibited caspase-3 activity to the basal level at the concentration of 10 mM. This study therefore demonstrated that A¥â induced cellular death might be prevented by KAG from butterbur leaves by the suppression of ROS and the subsequent recovery of apoptotic cell death.
|
|
KEYWORD
|
|
butterbur (Petasites japonicus), Alzheimer¡¯s disease, ¥â-amyloid peptide (A¥â), apoptosis, reactive oxygen species (ROS)
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|